書誌事項
- タイトル別名
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- Quantification of NADH-Cytochrome b5 Reductase (Metmyoglobin-Reducing Enzyme) in Bovine Skeletal Muscle by an Immunoblotting Assay
- Quantification of NADH Cytochrome b5 Re
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抄録
Quantitative determination method of NADH-cytochrome b5 reductase (me- tmyoglobin-reducing enzyme) in bovine skeletal muscle was developed by combining SDS-PAGE and immunoblotting techniques. Recoveries of the enzyme by this assay ranged from 90.0 to 94.4%. The content of NADH-cytochrome b5 reductase in bovine skeletal muscle was estimated to be 13.8±2.6(9.0-18.2)μg/g tissue (n=19). Also, a quantitative assay for determining cyto- chrome b5 (an electron transfer mediator for metmyoglobin reduction) was similarly established and cytochrome b5 content in bovine skeletal muscle was estimated to be 59.0±20.9μg/g tissue. The quantitative data of NADH-cytochrome b5 reductase in this study supported our previous hypothesis that the enzyme plays a critical role in preventing metmyoglobin accumulation in muscles.
収録刊行物
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- 日本畜産学会報
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日本畜産学会報 68 (1), 29-33, 1997
公益社団法人 日本畜産学会
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詳細情報 詳細情報について
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- CRID
- 1390282680171781504
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- NII論文ID
- 10024585652
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- NII書誌ID
- AN00195188
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- ISSN
- 18808255
- 00215309
- 1346907X
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- NDL書誌ID
- 4129887
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- 本文言語コード
- ja
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- データソース種別
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- JaLC
- NDL
- Crossref
- CiNii Articles
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- 抄録ライセンスフラグ
- 使用不可