A correlation between the superoxide anion scavenging capacity of antioxidants and their antioxidant capacity as measured by the galvinoxyl or DPPH method

DOI Web Site 17 References
  • Tohaya Yumiko
    Division of Life Science and Medical Engineering, University of East Asia
  • Ochiai Tameichi
    Division of Life Science and Medical Engineering, University of East Asia

Bibliographic Information

Other Title
  • 抗酸化物質のスーパーオキシド消去能とガルビノキシル法またはDPPH法で測定した抗酸化能との相関

Search this article

Abstract

The superoxide anion scavenging capacity of 11 antioxidants (AH) was observed, and the results were compared with the antioxidant capacity as measured by DPPH and galvinoxyl methods. The superoxide anion was generated by the xanthine/xanthine oxidase system. 4-[3-(4-iodophenyl)-2-(4- nitrophenyl)-2H-5-tetrazolio]-1,3-benzene disulfonate sodium salt (WST-1) was used as a probe of the superoxide anion. The color development rates of WST-1 were measured under various [AH]/[WST-1] ratios, and the data obtained were analyzed according to the following formula. R=ΔA0/ΔA=1+(kA/kW)*([AH]/[WST-1]), where ΔA0 and ΔA are the color development rates of WST-1 in the absence and presence of antioxidants, respectively, and kA and kW are the rate constants of the reaction of superoxide anion with the antioxidants and WST-1. The slope kA/kW calculated from the linear regression of the plot of [AH]/[WST-1] vs. V0/V, indicated the relative superoxide anion scavenging capacity of each antioxidant. The superoxide anion scavenging capacity decreased in the order of caffeic acid, n-propyl gallate, 7,8-dihydroxyflavone, gallic acid, catechin, pyrogallol, quercetin, L-ascorbic acid, BHA, 4-hydroxycoumarin and ferulic acid. Correlation coefficients between the superoxide anion scavenging capacity and the antioxidant capacity as measured by the galvinoxyl or DPPH method were as low as 0.448 (galvinoxyl method) and 0.368 (DPPH method), and it was shown that the antioxidant capacity measured by DPPH method or galvinoxyl method does not necessarily reflect the scavenging capacity of superoxide anion.

Journal

References(17)*help

See more

Keywords

Details 詳細情報について

Report a problem

Back to top