Pharmacokinetics of orally administered alginate oligosaccharides in the marine fish, sevenband grouper (<i>Epinephelus septemfasciatus </i>), and the possible influence on fish immune system

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  • Nishikawa Toru
    Nagasaki Prefectural Institute for Environment Research and Public Health Graduate School of Science and Technology, Nagasaki University
  • Yokose Takeshi
    Nagasaki Prefectural Institute for Environment Research and Public Health Graduate School of Science and Technology, Nagasaki University
  • Miyaki Kadoo
    Nagasaki Prefectural Institute of Fisheries
  • Kadomura Kazushi
    Nagasaki Prefectural Institute of Fisheries
  • Tsukiyama Yosuke
    Nagasaki Prefectural Institute of Fisheries
  • Yamamoto Yoshiko
    Division of Biochemistry, Faculty of Fisheries, Nagasaki University
  • Yamaguchi Kenichi
    Graduate School of Science and Technology, Nagasaki University Division of Biochemistry, Faculty of Fisheries, Nagasaki University
  • Oda Tatsuya
    Graduate School of Science and Technology, Nagasaki University Division of Biochemistry, Faculty of Fisheries, Nagasaki University

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Other Title
  • マハタへの経口投与におけるアルギン酸オリゴ糖の吸収性と免疫機能への影響
  • Pharmacokinetics of orally administered alginate oligosaccharides in the marine fish, sevenband grouper (Epinephelus septemfasciatus), and the possible influence on fish immune system

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We have previously developed a sensitive and simple liquid chromatography/tandem mass spectrometry (LC-MS/MS) method for the detection of alginate oligosaccharides (AOs) in an in vivo mouse model. By this method, in this study, we detected AOs in the plasma from marine fish sevenband grouper which had been fed diets containing AOs. Based on a calibration curve made with an AO trimer peak area as a standard, the plasma concentration of AOs was estimated to be 6.5μg/mL and 7.9μg/mL at 6h after single administration of the diets containing AOs at 5% or 10% (w/w), respectively. Relatively high level of AOs in the plasma was maintained for at least 54h. Sevenband groupers, which had been fed control diet, or AO-containing diet for 2 weeks in rearing tanks, were released into the net placed in the coastal water where the risk of prevalence of infectious diseases especially VNN had been increasing. After 60d culture with control or AO-containing diet, the survival rate of the fish fed diet containing AOs was significantly higher than that of the fish fed control diet. Bio-Plex bead assay, that can detect 23 mouse cytokines at the same time, suggested that intraperitoneal administration of AOs induced the increase in cytokines especially TNF-α in the plasma of sevenband grouper. Our results suggest that orally administered AOs are absorbed by digestive organs, and are retained in the plasma for relatively long time. The long-lasting retention of absorbed AOs in the plasma may be attributed to the activation of fish immune system.

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