天然増粘安定剤キダチアロエ抽出物の確認試験法の確立

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タイトル別名
  • Identification Test of Aloe Extract from <i>Aloe arborescens</i>, a Natural Thickening Stabilizer
  • テンネン ゾウネン アンテイザイ キダチアロエ チュウシュツブツ ノ カクニン シケンホウ ノ カクリツ

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抄録

Aloe extract product is an extract from the leaves of Aloe arborescens Mill., which is used as a natural thickening stabilizer in Japan. In order to establish the identification method of aloe extract product, we investigated the characteristic low molecular constituents such as anthrone, chromone and phenyl pyrone derivatives by LC/MS and TLC. We confirmed that aloe extract was different in the constituents from JP grade aloe and reagent grade aloin. Aloenin was detected only in aloe extract from A. arborescens. The detection of aloenin made it possible to distinguish aloe extract from JP grade aloe and reagent grade aloin. The amounts of aloenin, barbaloin, and isobarbaloin in the aloe extract were determined by using the absolute calibration method, and their contents were 2.0%, 0.8%, and 0.7%, respectively. The correction factor (CF) was derived from the calibration curves of barbaloin and aloenin, and it was confirmed that the content of aloenin was also calculated from the calibration curve of barbaloin and the correction factor (CF) even if aloenin standard could not be found in reagent markets.

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