An Improved System for Selection of Forward Mutations in an Escherichia coli supF Gene Carried by Plasmids.
-
- OBATA FUMIKO
- Biological Institute, Graduate School of Science, Tohoku University
-
- NUNOSHIBA TATSUO
- Biological Institute, Graduate School of Science, Tohoku University
-
- HASHIMOTO-GOTOH TAMOTSU
- Department of Biochemistry and Molecular Genetics, Kyoto Prefectural University of Medicine
-
- YAMAMOTO KAZUO
- Biological Institute, Graduate School of Science, Tohoku University
書誌事項
- タイトル別名
-
- Improved System for Selection of Forward Mutations in an Escherichia coli supF Gene Carried by Plasmids
- 公開日
- 1998
- 資源種別
- journal article
- DOI
-
- 10.1269/jrr.39.263
- 公開者
- Journal of Radiation Research 編集委員会
この論文をさがす
説明
An improved system to examine forward mutations that occurred in the supF gene of Escherichai coli carried on a multicopy plasmid is described. The system was validated by measuring spontaneous mutations of supF plasmids propagated in wild-type, recA- and mutM- mutY- E. coli strains, the mutation frequencies of which were 1.3 × 10-7, 6.3 × 10-7 and 1.5 × 10-6, respectively. Sequence analysis of the supF mutant plasmids revealed that G:C→T:A and G:C→C:G transversions dominated. This improved system allows rapid scoring and sequencing forward mutations in the supF gene, thus permitting its use as a genetic target for repair and mutagenesis studies in bacteria and mammalian cells.
収録刊行物
-
- Journal of Radiation Research
-
Journal of Radiation Research 39 (4), 263-270, 1998
Journal of Radiation Research 編集委員会
- Tweet
キーワード
詳細情報 詳細情報について
-
- CRID
- 1390282680192038528
-
- NII論文ID
- 110002342231
-
- NII書誌ID
- AA00705792
-
- COI
- 1:CAS:528:DyaK1MXhsFSjsbo%3D
-
- ISSN
- 13499157
- 04493060
- http://id.crossref.org/issn/04493060
- https://id.crossref.org/issn/04493060
-
- PubMed
- 10196781
-
- 本文言語コード
- en
-
- 資料種別
- journal article
-
- データソース種別
-
- JaLC
- NDLサーチ
- Crossref
- PubMed
- NDLデジコレ（旧NII-ELS）
- CiNii Articles
- OpenAIRE
-
- 抄録ライセンスフラグ
- 使用不可
