In vitro pain assay in cultured human iPSC-derived sensory neurons using MEA system

DOI
  • Odawara Aoi
    Gluaduate school of engineering, Tohoku Institute of Technology Research Institute of Electrical Communication, Tohoku University JSPS
  • Iida Takuya
    Gluaduate school of engineering, Tohoku Institute of Technology
  • Matsuda Naoki
    Gluaduate school of engineering, Tohoku Institute of Technology
  • Suzuki Ikuro
    Gluaduate school of engineering, Tohoku Institute of Technology

Bibliographic Information

Other Title
  • MEAシステムを用いたヒトiPS細胞由来感覚ニューロンのin vitro痛み応答計測

Description

<p>Sensory neurons are pain-related neurons and have a variety of receptors that are activated by chemical, thermal, and mechanical stimuli. We evaluated the physiological responses aginst pain-related molecules and temperature in cultured human induced pluripotent stem cell (hiPSC)-derived sensory neurons using multi-electrode array (MEA) system. Capsaicin, menthol and AIT induced significant changes of the firing rate and concentration-dependent responses. We also found the difference of response speed and duration depending on the type of molecules, and that neurons were classified into 27 patterns based on responses against 3 molecules. Furthermore, temperature elevation increased the number of firings and it showed the largest increase at 43 degrees. Moreover, we also confirmed the expression of Nav1.7, TRPV1, and TRPA1 receptors. These results suggested that electrophysiological measurements in hiPSC-derived sensory neurons using a MEA system may be beneficial for clarifying the functions of human neurons in pain research and for drug screening applications.</p>

Journal

Details 詳細情報について

  • CRID
    1390282680246281344
  • NII Article ID
    130006076960
  • DOI
    10.11239/jsmbe.55annual.231
  • ISSN
    18814379
    1347443X
  • Text Lang
    ja
  • Data Source
    • JaLC
    • CiNii Articles
  • Abstract License Flag
    Disallowed

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