Construction of 3D cardiac tissue with patterned vascular structure by inkjet printer head

DOI
  • Miyaoka Atsushi
    Biomedical Research Department, Ricoh Institute of Future Technology, Ricoh Graduate School of Frontier Biosciences, Osaka University
  • Tsukamoto Yoshinari
    Graduate School of Frontier Biosciences, Osaka University
  • Yokoyama Junya
    Department of Cardiovascular surgery, Osaka University
  • Hatada Shigeo
    Biomedical Research Department, Ricoh Institute of Future Technology, Ricoh
  • Kamono Shunpei
    Biomedical Research Department, Ricoh Institute of Future Technology, Ricoh
  • Takagi Daisuke
    Biomedical Research Department, Ricoh Institute of Future Technology, Ricoh
  • Tano Takanori
    Biomedical Research Department, Ricoh Institute of Future Technology, Ricoh
  • Akashi Mitsuru
    Graduate School of Frontier Biosciences, Osaka University

Bibliographic Information

Other Title
  • インクジェット技術による血管網パターニングを目指した三次元心筋組織作製の検討

Description

<p>In currently available construction methods for artificial tissue, the internal structure of 3-D tissue is dependent on self- organization of cells. We hoped to provide a technology that enables more precise control of such internal structure in engineered 3-D tissue. In this report, we use cardiomyocytes differentiated from human induced pluripotent stem cells (iPS-CM), normal human arterial cardiac fibroblasts (NHCF) and human cardiac microvascular endothelial cells (HMVEC) as the models to construct multi-layered 3-D tissue. We aimed to deposit HMVECs in the 3-D tissue comprised of iPS-CM/NHCF using our inkjet printer head. In order to make the structure and the function closer to the natural tissue, we developed a new cell coating method using extracellular matrix (ECM). The result of this study demonstrates the potential of our new technologies in inkjet head and ECM-based cell coating to engineer functional 3-D tissue with improved precision in its internal structure.</p>

Journal

Details 詳細情報について

  • CRID
    1390282680246841216
  • NII Article ID
    130006077196
  • DOI
    10.11239/jsmbe.55annual.427
  • ISSN
    18814379
    1347443X
  • Text Lang
    ja
  • Data Source
    • JaLC
    • CiNii Articles
  • Abstract License Flag
    Disallowed

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