Quadrat method for measurement of injurious effects of Hypoxylon truncatum on bed logs used for shiitake (Lentinula edodes) cultivation

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  • TSUNODA Mitsutoshi
    Research Planning and Coordination Department, Forestry and Forest Products Research Institute (FFPRI)
  • TANIGUCHI Minoru
    Department of Applied Microbiology, Forestry and Forest Products Research Institute (FFPRI)

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Other Title
  • 区画法によるシイタケほだ木のクロコブタケ被害測定
  • クカクホウ ニ ヨル シイタケ ホダギ ノ クロコブタケ ヒガイ ソクテイ

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Description

Hypoxylon truncatum grows in the bed logs used for shiitake (Lentinula edodes) cultivation and forms perithecial stromata on them. The surface of these bed logs was divided into 160 quadrats by 8 vertical and 20 transverse equal interval grid lines, and the presence of stromata of H. truncatum in each quadrat was examined to estimate the injurious effects of H. truncatum on the bed logs. The ratio of quadrat to stroma was defined as the ratio of the sum of quadrats with stromata to the total number of quadrats. Each bed log was cut transversely at equal intervals into twenty disks and the area occupied by H. truncatum on the section was identified based on dark areas surrounded by a dark line. A positive correlation was apparent between the ratio of quadrat to stroma and the ratio of the total area occupied by H. truncatum to the total cross-sectional area of the bed log. The ratio of quadrat to stroma was divided into two or three grades, and the bed logs having each grade were used for further tests of shiitake cultivation. The fruiting body yield of shiitake was negatively correlated with the ratio of quadrat to stroma.

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