加熱ハブ毒による能動免疫に関する実験的研究

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書誌事項

タイトル別名
  • EXPERIMENTAL STUDIES ON THE ACTIVE IMMUNIZATION OF GUINÉA PIGS BY HEATED HABU SNAKE VENOM
  • カネツ ハブ ドク ニ ヨル ノウドウ メンエキ ニ カンスル ジッケンテキ ケンキュウ
公開日
1965
DOI
  • 10.2974/kmj1951.15.309
公開者
北関東医学会

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説明

Immunization with heated Habu Snake venom was attempted on guinea pigs.<BR>1. Freund's incomplete adjuvant was added to equivalent volumes of supernatants of heated Habu Snake venom (50mg/1.0ml in 0.85% saline), and 1. 0ml of the mixture was subcutaneously injected into the back of guinea pigs. One week after, 0. 1ml of the heated venom (500γ/0.1ml) was injected one time as additional immunization (booster) <BR>2. When, at two to twelve week after the final immunization, the animals were challenged by injecting 500γ or less of the crude venom into the femoral muscles, the grade and extension of the local damage in them were 1/3 to 1/4 as small as those in the nonimmunized animals.<BR>3. When 0. 1ml of the sera obtained from the immunized animals was mixed in vitro with various amounts of the crude and the heated venoms and the mixture were injected intramuscularly into the legs of mice, they depressed the local pathological changes which were visible after injection of 64γ to 34γ of the crude venom or heated venom obtained from 500γ to 250γ of the crude venom.

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