ディスク電気泳動法による穀類の可溶性たん白質の分析

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タイトル別名
  • Disc Electrophoresis of the Soluble Protein Fractions of Cereals
  • ディスク デンキ エイドウホウ ニヨル コクルイ ノ カヨウセイ タンパクシツ

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説明

Extracts of polished rice (Oryza sativa var. japonica), pressed barley (Hordeum vulgare L. emend LAM) and wheat flour (Triticum aestivum L.) were prepared by three procedures using distilled water and 50mM Tris-HCl buffer (pH 8.0): (A) the first extraction by gentle stirring, (B) the second extraction by homogenizing the residual material of the (A), and (C) the extraction from the homogenate of boiled material. Great differences in the disc electrophoretic patterns of soluble protein fractions of (A), (B) and (C) were observed between the employed solvents.<BR>Even when the same solvent is used, the first and the second extract of each material gave somewhat different patterns. High concentrations of soluble proteins were found to be present in the extracts from boiled materials. Each sample showed a pattern peculiar to the species.

収録刊行物

  • 栄養と食糧

    栄養と食糧 29 (2), 127-130, 1976

    公益社団法人 日本栄養・食糧学会

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