わい性ネピアグラスにおける腋芽生長点からの多芽体形成と効率的な苗生産

DOI
  • Nafiatul UMAMI
    宮崎大学大学院農学工学総合研究科 Faculty of Animal Science, Gadjah Mada University
  • 権藤 崇裕
    宮崎大学フロンティア科学実験総合センター
  • 石垣 元気
    宮崎大学フロンティア科学実験総合センター
  • Mohammad Mijanur RAHMAN
    Institute of Biological Sciences, Faculty of Science, University of Malaya
  • 明石 良
    宮崎大学フロンティア科学実験総合センター

書誌事項

タイトル別名
  • <b>Efficient nursery production and multiple-shoot clumps formation from shoot tiller-derived shoot apices of dwarf napiergrass (Pennisetum purpur</b><b>eum Schumach. ) </b>

抄録

<tt>The objective of this study was to improve an </tt><tt>in vitro </tt><tt>tissue culture system for the dwarf napiergrass (</tt><tt>Pennisetum purpureum </tt><tt>Schumach. ). Additionally, it was needed special treatments for acclimatization, and carried the risks of somaclonal variation and physiological aberrations. Shoot apices as initial explants were isolated aseptically from shoottillers, and cultured on </tt><tt>in vitro </tt><tt>MS (Murashige and Skoog, 1962) medium containing 3.0% sucrose and 0.3% phytagel. The most effective phytohormone treatment for multiple-shoot clumps (MSCs) induction was 0.1 mg L</tt><tt>-1 </tt><tt>2,4-D plus 2.0 mg L</tt><tt>-1 </tt><tt>BAP. The addition of 50 </tt>μ<tt>M CuSO4 could increase the percentage of MSCs proliferation. Plant regeneration frequency was achieved up to 84% by culturing the MSCs on solid MS medium containing 0.1 mg L</tt><tt>-1 </tt><tt>NAA and 2.0 mg L</tt><tt>-1 </tt><tt>BAP. All regenerants were successfully grown up in the soil. Compared to control plants, </tt><tt>in vitro </tt><tt>regenerated plants did not reveal any significant difference (</tt><tt>P </tt><tt>> 0.05) on morphological characteristics and DNA content. The results of this study suggest that improved protocols for </tt><tt>in vitro </tt><tt>propagation would provide high quality nursery plant production of dwarf napiergrass in </tt><tt>grassland field. </tt>

収録刊行物

キーワード

詳細情報 詳細情報について

  • CRID
    1390282680320476416
  • NII論文ID
    130004555651
  • DOI
    10.11461/jwaras.55.121
  • ISSN
    21851670
    2185081X
  • 本文言語コード
    en
  • データソース種別
    • JaLC
    • CiNii Articles
  • 抄録ライセンスフラグ
    使用不可

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