Bacterial Flora of Piglets under Specific Pathogen Free Conditions

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  • SPF (Specific Pathogen Free) ブタの保有する細菌叢について
  • SPF Specific Pathogen Free ブタ ノ ホユウ スル サイキンソウ ニ ツイテ

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Description

An isolation facility for the production and maintenance of specific pathogen free pigs was designed at National Institute of Animal Health, Kodaira, Tokyo. A total 82 piglets in 9 experi-ments obtained by hysterectomy was transfer-ed into the air conditioning room, and they were raised and maintained by technics designed to protect them from microbial contamination, i, e, all supplies were sterilized before use, and personnel put on a special designed sterile clothes. These isolated piglets were examined for bacte-rial contamination for 42 days.<BR>1. Escherichia coli, Enterobacter cloacae, bacilli, micrococci, and pseudomonas were frequ-ently recovered from the feces of the isolated piglets during the 4 th day of operation. How-ever, all these bacterial genera except Escheri-chia coli, subsequently disappeared. Later Streptococcus faecalis and bacteroides were also frequently isolated (Table 2) . The mean bacteri-al counts per gram of the feces of the isolated piglets at 42 nd day after operation were as follows: Escherichia coli, 109.5; Streptococcus fae-calis, 108.8; Bacteroides, 108.4; and Proteus vulgaris, 106.1 (Fig.1) .<BR>2. Death with bacteremia occured most often in the isolated piglets between 3 rd to 5 th day after operation. Escherichia coli, Enterobacter cloacae, or Klebsiella, in profuse and apparently pure culture, isolated from the blood or organs of the dead piglets (Table 3) .

Journal

  • EXPERIMENTAL ANIMALS

    EXPERIMENTAL ANIMALS 18 (1), 1-6, 1969

    Japanese Association for Laboratory Animal Science

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