Evaluation and regulation cellular/organ functions by optic technology

DOI
  • Ozaki Michitaka
    Department of Biological Response and Regulation, Faculty of Health Sciences, Hokkaido University Laboratory of Molecular and Functional Bio-Imaging, Faculty of Health Sciences, Hokkaido University
  • Haga Sanae
    Department of Biological Response and Regulation, Faculty of Health Sciences, Hokkaido University
  • Ozawa Takeaki
    Department of Chemistry, The University of Tokyo
  • Morita Naoki
    Molecular and Biological Technology Research Group, National Institute of Advanced Industrial Science and Technology
  • Hamada Toshiyuki
    Department of Biological Response and Regulation, Faculty of Health Sciences, Hokkaido University

Bibliographic Information

Other Title
  • 光技術を用いた臓器・細胞機能評価と臨床応用

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Abstract

To develop an effective organ/cell preservation method and to monitor post-transplant graft function continuously and non-invasively, an innovative optic technology to visualize cell/organ function seems to be useful. We have developed some optic probes to visualize regulated cell death (apoptosis, necroptosis, pyroptosis), redox states and cellular stresses, pH and cellular antigens in deeper lesions of the organ. In the hepatic ischemia/reperfusion model of mice, we successfully imaged liver oxidative stress (by redox-sensitive GFP) and apoptosis (by caspase-3 activity) non-invasively and chronologically in a single mouse. We also developed a unique tool to visualize intracellular pH and succeeded in imaging dynamic changes of pH in a mouse posterior limb ischemia/reperfusion model. We are also developing the new devices for tissue/organ imaging. We are trying to monitor the optic signals chronologically and track the lesions in the body by developing light sensor and multiple CCD camera system, which can be used in endoscopic examination and surgical operation. It is still on a way, but this kind of technology will definitely provide a new avenue toward effective and non-invasive surgical therapy in the future.

Journal

  • Organ Biology

    Organ Biology 24 (2), 207-211, 2017

    The Japan Society for Organ Preservation and Biology

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