Characterization of promoter of <I>Wiv-1</I> (<I>Lin6</I>) gene for wound-inducible cell wall-bound acid invertase of tomato.

DOI
  • Ohyama Akio
    National Institute of Vegetable and Tea Science (NIVTS), National Agricultural Research Organization (NARO)
  • Suwabe Keita
    National Institute of Vegetable and Tea Science (NIVTS), National Agricultural Research Organization (NARO)
  • Nunome Tsukasa
    National Institute of Vegetable and Tea Science (NIVTS), National Agricultural Research Organization (NARO)
  • Hirai Masashi
    National Institute of Vegetable and Tea Science (NIVTS), National Agricultural Research Organization (NARO) Graduate School of Agricultural Science, Kyoto Prefectural University
  • Fukuoka Hiroyuki
    National Institute of Vegetable and Tea Science (NIVTS), National Agricultural Research Organization (NARO)

書誌事項

公開日
2003
DOI
  • 10.14841/jspp.2003.0.604.0
公開者
日本植物生理学会

説明

A genomic clone of Wiv-1 gene for wound-inducible cell wall-bound acid invertase was isolated from tomato. The 5' upstream region (4kbp) of the gene contained some regulatory elements for phloem-specific expression. This region was fused to the beta-glucuronidase (GUS) gene, and then the chimeric gene was introduced to tobacco. In leaves of the transgenic tobacco, GUS activity continued to increase for 96 h after wounding. A strong GUS staining was observed in pollens and vascular tissues of stems close to petioles in transgenics grown in a greenhouse. These data suggest that the cell wall-bound enzyme encoded by Wiv-1 involves in the regulation of transport of sucrose in leaves and in the sink activity of pollens. In addition, results of some experiments for the induction of Wiv-1 gene expression by sugars will be reported as well.

収録刊行物

詳細情報 詳細情報について

  • CRID
    1390282680606103552
  • NII論文ID
    130006989823
  • DOI
    10.14841/jspp.2003.0.604.0
  • 本文言語コード
    en
  • データソース種別
    • JaLC
    • CiNii Articles
  • 抄録ライセンスフラグ
    使用不可

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