Overcoming artifacts in pathological specimens (First report): Study of the mechanism and elimination of chattering

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  • 病理標本のアーチファクトの克服（第1報）―チャタリングのメカニズムとその解消方法の検討―

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Chattering is a troublesome artifact showing parallel vibration against a blade line when we cut hard samples such as uterine myomas. We attempted to clarify what condition easily causes this artifact and how to prevent it. We found that the thinner the section, the faster the velocity of slicing, and the lower the temperature of sample blocks, the easier for chattering to occur. From these results, we consider that chattering may occur depending on the slicing technique. It may also be due to complex factors arising from vibrations of the blade slicing a tissue that is contracted and hardened owing to its dehydration and heating during paraffin infiltration for tissue block preparation. Rehydration of the surface of blocks by wet compress or by using a mist humidifier was effective in preventing chattering. Rehydration can soften a tissue and prevent vibration of the slicing blade.

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