Evaluation of antinuclear antibody test by chemiluminescent enzyme immunoassay (CLEIA)

KOMURA Naoko, KOMATSU Kazunori, KONO Hajime, FURUKAWA Taiji

doi:10.14932/jamt.16-3

Bibliographic Information

Other Title

CLEIA法を原理とする抗核抗体測定試薬の評価

Description

<p>Detection of the antinuclear antibody (ANA) is widely used for the screening of collagen diseases. Although indirect immunofluorescence (IIF) assay is considered as a standard test, it has disadvantages in terms of its specificity and rapidity. We performed basic and clinical evaluation of a new ANA assay by chemiluminescent enzyme immunoassay (CLEIA). The basic performance of the assay was within acceptable level. Its clinical sensitivity was 91.5% and its specificity was 74.2%, which are higher than those of the IIF assay. The measurement time of CLEIA was 19 minutes, which was shorter than that of the conventional method. On the basis of these results, it is considered that this method is useful for the screening of connective tissue diseases.</p>

Journal

Japanese Journal of Medical Technology

Japanese Journal of Medical Technology 65 (6), 629-635, 2016

Japanese Association of Medical Technologists

Keywords

Details 詳細情報について

CRID: 1390282680718450176

NII Article ID: 130005289376

DOI: 10.14932/jamt.16-3

ISSN: 21885346; 09158669

Text Lang: ja

Data Source

JaLC
CiNii Articles

Abstract License Flag: Disallowed

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