CCR4 and CCR5 as amarker of Th2 and Th1 Cells in human peripheral blood lymphocytes

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  • TAKANASHI TAKESHI
    Department of Pediatrics, Juntendo University School of Medicine
  • MATSUDA HIRONORI
    Department of Immunology, Juntendo University School of Medicine
  • YAGITA HIDEO
    Department of Immunology, Juntendo University School of Medicine
  • OKUMURA KO
    Department of Immunology, Juntendo University School of Medicine

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  • 人末梢血リンパ球中のTh2・Th1細胞　マーカーとしてのCCR4・CCR5

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Chemokine receptors play important roles in controlling the migration of lymphocytes to lymphoid and non-lymphoid tissues. Recent studies have shown that CCR4 is preferentially expressed on type 2 helper T (Th2) cell lines producing interleukin-4 (IL-4) and IL-13, and CCR5 is expressed on type 1 helper T (Th1) cell lines producing interferon-γ (IFN-γ). In this study, we investigated how CCR4 and CCR5 are expressed on peripheral blood T lymphocytes from 33 healthy individuals. CCR4 and CCR5 marked two distinct subsets of memory T lymphocytes expressing CD45RO in a mutually exclusive manner. Among CD4+ memory T lymphocytes, 10-20% expressed CCR4 but not CCR5 and 15-25% expressed CCR5 but not CCR4. In contrast, the majority (50-70%) of CD8+ memory T lymphocytes expressed CCR5 but not CCR4. After in vitro stimulation, CCR4+ cells isolated from memory T lymphocytes predominantly contained IL-4-producing cells (15%) over IFN-γ-producing cells (2%) and, conversely, CCR5+cells predominantly contained IFN-γ-producing cells (60%) but only a few IL-4-producing cells (3%). These results indicated that CCR4 and CCR5 can be useful surface markers of Th2 and Th1 cells in human peripheral blood T lymphocytes.

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