Prediction of Feed Composition and Antioxidant Activity of Sorghum Grain by Near Infrared Reflectance Spectroscopy

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  • 近赤外分光分析によるソルガム穀実の飼料成分および抗酸化活性の推定
  • キンセキガイ ブン コウブンセキ ニ ヨル ソルガムコクミノル ノ シリョウ セイブン オヨビ コウサンカ カッセイ ノ スイテイ

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Abstract

In this study, we established the calibration equation for determining the feed composition and antioxidant activity of powdered and non-powdered sorghum grain. The results showed that near-infrared reflectance (NIR) analysis of powdered grain enabled the rough prediction of the concent-rations of crude protein (CP), crude fat (EE), acid detergent fiber (ADFom), neutral detergent fiber (aNDFom), and tannin, and 1,1-diphenyl-2-picrylhydrazyl (DPPH) radical-scavenging activity, with computation of the validation correlation and standard deviation/square error of prediction values (RPD). These values were determined to be as follows: CP, 0.87 and 2.48 ; EE, 0.85 and 2.53 ; ADFom, 0.86 and 2.63 ; aNDFom, 0.69 and 1.54 ; tannin content, 0.90 and 2.95 ; and DPPH radical-scavenging activity, 0.87 and 2.63. Furthermore, the results for NIR analysis of non-powdered grain were as follows : CP, 0.60 and 1.57 ; EE, 0.61 and 1.60 ; ADFom, 0.66 and 1.66 ; aNDFom, 0.57 and 1.46 ; tannin content, 0.89 and 2.92 ; and DPPH radical-scavenging activity, 0.87 and 2.72. These results indicate that the concentrations of CP, EE, ADFom, aNDFom, and tannin, and DPPH radical-scavenging activity in powdered sorghum grain as well as the tannin content and DPPH radical-scavenging activity in non-powdered sorghum grain were good enough for rough screening by using NIR techniques.

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