動物組織のリポキシゲナーゼの研究 : II.特異的阻害剤と酵素免疫測定法の開発

DOI Web Site

書誌事項

タイトル別名
  • Studies on Mammalian Lipoxygenases : II. Development of Selective Inhibitor and Enzyme Immunoassay
  • 動物組織のリポキシゲナーゼの研究-2-特異的阻害剤と酵素免疫測定法の開発
  • ドウブツ ソシキ ノ リポキシゲナーゼ ノ ケンキュウ 2 トクイテキ ソガイ

この論文をさがす

抄録

In view of pathological roles of leukotrienes a potent selective inhibitor of arachidonate 5-lipoxygenase was developed on the basis of an earlier finding that flavonoids suppressed anaphylactic reactions. 5-Hexyloxy-3',4'-dihydroxy-6,7-dimethoxyflavone 4'-disodium phosphate as a 5-lipoxygenase inhibitor inhibited in vivo production of peptidoleukotrienes in the gerbil brain subjected to ischemia and reperfusion. The monoclonal antibodies raised against 12- and 5-lipoxygenases and cyclooxygenase were utilized for development of peroxidase-linked immunoassays of these enzymes, which allowed a quantitative determination of the enzyme amount rather than the enzyme activity. The method was applied to lipoxygenase screening in various animal tissues. A clinical case of cyclooxygenase abnormality was analyzed by the enzyme immunoassay.

収録刊行物

  • ビタミン

    ビタミン 62 (2), 61-67, 1988

    公益社団法人 日本ビタミン学会

キーワード

詳細情報 詳細情報について

問題の指摘

ページトップへ