A Simple Method for Determination of Vitamin D in Foods by Ethyl Acetate/n-Hexane Extraction : High Performance Liquid Chromatography

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  • 酢酸エチル・ヘキサン抽出-HPLCによる食品中のビタミンD定量法
  • サクサン エチル ヘキサン チュウシュツ HPLC ニ ヨル ショクヒンチュウ

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Description

A method for determination of vitamin D in foods, including saponification and triplicate extraction system with ethyl acetate/n-hexane (1 : 9) and high performance liquid chromatography is described. Recoveries of added vitamin D from lard and egg white were 97.1 % and 102.6 %, respectively. The values of relative standard deviations in five replicate determinations for three kinds of modified milk powders and mushroom "Shiitake" were 0.30, 3.85, 1.98 and 2.60 %, respectively.

Journal

  • VITAMINS

    VITAMINS 68 (5-6), 303-307, 1994

    THE VITAMIN SOCIETY OF JAPAN

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