Determination of Theophylline in Biological Fluids by High-Performance Liquid Chromatography

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  • 高速液体クロマトグラフ法による体液中のテオフィリンの定量
  • コウソク エキタイ クロマトグラフホウ ニヨル タイエキチュウ ノ テオフィリ

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Abstract

High performance liquid chromatographic method for the determination of theophylline in biological fluids (serum, saliva and urine) was developed. Theophylline in biological fluids was adsorbed on a diatom earth column, followed by elution with a mixture of chloroform-isopropanol (95 : 5). After evaporation of the solvent, the residue was dissolved in water, injected onto a HPLC column packed with an ODS stationary phase eluted at 40°C with a mixture of 0.1M phosphate buffer (pH 5.3)-acetonitrile (9 : 1) and detected at 273 nm. 8-Chlorotheophylline was used as an internal standard. The recovery of theophylline from serum by this method was 100.1%±1.76% (SD ; n=5), Other xanthine derivatives (theobromine and caffeine) were seperated under the same conditions.

Journal

  • YAKUGAKU ZASSHI

    YAKUGAKU ZASSHI 101 (10), 955-959, 1981

    The Pharmaceutical Society of Japan

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