EFFECT OF TRYPSIN ON VIRAL SUSCEPTIBILITY OF VERO CELL CULTURES-CERCOPITHECUS KIDNEY LINE

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Addition of dilute trypsin to the maintenance medium of Vero cell cultures made them far more susceptible to certain strains of parainfluenza type 1 (HVJ) and influenza A and B viruses. These findings are in line with those already reported on parainfluenza type 1 (HA-2) and type 4 viruses. To other virus strains belonging to myxo, picorna, reo, arbo, adeno, herpes, papova and poxvirus groups, the susceptibility was not enhanced by this manipulation. The difference of infectivity titers between those measured in Vero cell cultures under the trypsin-added condition (Vrt) and the ordinary ones (Vro) was taken as a criterion for the enhancement. In the serial passage tests of selected viruses in Vrt, most of viruses with the above titer-enhancement was proved transferable in the trypsin-added system, but remained nontransferable in Vro even after the passages in Vrt. Consequently, a possible emergence and selection of variants specially adapted to Vero cells is unlikely to occur in the Vrt system. Therefore, the present observations were considered on the basis of modified viral susceptibility of the cell culture.

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