Analysis of mariner-like elements in the smaller tea tortrix, Adoxophyes honmai and the summer fruit tortrix, Adoxophyes orana fasciata (Lepidoptera: Tortricidae).
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- Yoshiyama Mikio
- Institute of Agriculture and Forestry, University of Tsukuba
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- Honda Hiroshi
- Institute of Agriculture and Forestry, University of Tsukuba
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- Noguchi Hiroshi
- Department of Environmental Biology, National Institute of Agro-Environmental Sciences
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- Kimura Kiyoshi
- Department of Animal Genetics, National Institute of Animal Industry
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The mariner-like element (MLE) in two closely related leaf-roller moths, the smaller tea tortrix, Adoxophyes honmai and the summer fruit tortrix, Adoxophyes orana fasciata (Lepidoptera: Tortricidae) were surveyed. By a series of PCR and Southern blotting experiments, there were at least two types of MLEs in these two species. Sequence dissimilarity of each MLE suggests a possible polyphyletic origin and subsequent vertical inheritance within each species. In addition, a full-length MLE was isolated from a genomic library of A. honmai. This element, named Ahmarl, was 1,294 bp long with 27 bp inverted terminal repeats (ITRs). This element is apparently inactive due to a frameshift in the open reading frame (ORF) region, but a putative ORF could be observed by a single nucleotide removal, suggesting that the MLE in this species could have had the ability to transpose in the near past. Ahmarl may be suitable as a transformation vector with slight modification.
収録刊行物
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- Applied Entomology and Zoology
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Applied Entomology and Zoology 35 (3), 313-320, 2000
日本応用動物昆虫学会
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詳細情報 詳細情報について
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- CRID
- 1390282681220875648
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- NII論文ID
- 110001103429
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- NII書誌ID
- AA00543238
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- ISSN
- 1347605X
- 00036862
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- NDL書誌ID
- 5504989
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- 本文言語コード
- en
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- データソース種別
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- JaLC
- NDL
- Crossref
- CiNii Articles
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- 使用不可