Antitumor effect of orally administered extracts from fruit body of Grifola frondosa (maitake).
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We conducted a footpad swelling test to clarify whether a delayed type hypersensitivity (DTH) reaction against tumor antigen is potentiated by oral administration of D fraction extracted and purified from fruit body of Grifola frondosa (maitake), with a ratio of β-glucan to protein of 7: 3. When the antigen was injected in D fraction administered tumorbearing mice (D-mice), footpad swelling was observed, suggesting that a DTH reaction against the tumor antigen was potentiated by oral administration of D-fraction. Winn assay was carried out with whole spleen cells from D-mice and Lyt-2-spleen cells in which Lyt-2+ cells corresponding to cytotoxic T cells (CTL) were eliminated by treating with anti-Lyt-2 monoclonal antibody and complement. Even after CTL were eliminated, tumor neutralizing activity was at no time impaired, indicating that spleen cells other than CTL play a leading role in tumor cytotoxicity.<BR>We hypothesize from the above results, that a DTH reaction, manifested by the combined action of delayed hypersensitivity T cells (Tdh) and macrophages (MΦ), was potentiated by oral administration of D-fraction. Even when CTL are absent, there is a high degree of tumor involution. But D-fraction did not activate gut-associated lymphoid tissue (GALT) and Peyer's patch (PP), by oral administration.
CHEMOTHERAPY 38 (8), 790-796, 1990
Japanese Society of Chemotherapy