Purification and Some Properties of a Maltotriose-producing Amylase

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  • マルトトリオースを生成する新規アミラーゼの精製と酵素的性質
  • マルトトリオース オ セイセイスル シンキ アミラーゼ ノ セイセイ ト コウ

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A new amylase from Streptomyces griseus NA-468 was isolated and some properties were studied. The amylase was purified by means of acetone precipitation, CM-Sephadex C-50 column chromatography and Sephadex G-100 gel filtration, and a single band, which has the activity of producing maltotriose from starch was obtained on a polyacrylamide disc gel electrophoresis. The purified amylase showed maximal activity at 45°C and pH 5.6-6.0. The amylase hydrolyzed amylose completely, and produced 51% of maltotriose from waxy corn starch, though pullulan, β-cycrodextrin and waxy corn starch β-limit dextrin were not attacked. From the two-dimensional paper chromatogram of the digests of maltooligosaccharides, it was suggested that the amylase cleaved regularly the third glucosidic bonds from the nonreducing end of the substrates.

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