ホウレンソウプロトプラストからの植物体再生における品種間差異

DOI NDLデジタルコレクション 日本農学文献記事索引 Web Site Web Site 被引用文献2件 参考文献13件

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タイトル別名
  • Varietal Variations in Plant Regenerative Potential from Protoplasts in Spinach (Spinacia oleracea L.).
  • Varietal variations in plant regenerati

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抄録

The regenerative potential of seven Spinacia oleracea L. varieties in protoplast culture was compared. Although protoplasts were isolated from all cultivars, the yield of protoplast, plating efficiency (PE), and colony formation ratio were remarkably different among them. The highest PE and colony formation ratio were obtained from protoplasts of 'Ujo', followed by 'Jiromaru', 'Hoyo', and 'Minsterland', which exceeded those of 'Sapporo-oba', 'Viroflay', and 'Viking'. Shoots were regenerated by 'Ujo', 'Jiromaru', 'Hoyo', and 'Minsterland'. 'Hoyo' and 'Jiromaru' had the highest frequency ; no shoot developed from the calli of 'Sapporo-oba', 'Viroflay', and 'Viking'. The highest frequency of shoot regeneration was obtained in a media containing 1.0 or 10.0mg·liter-1 IBA, but at 10.0mg·liter-1 IBA or IAA, the shootlets were often vitrified and fasciated.

収録刊行物

  • 園芸学会雑誌

    園芸学会雑誌 67 (4), 503-506, 1998

    一般社団法人　園芸学会

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