Epidemiological Survey of Streptococcus mutans among Japanese Children

DOI Web Site Web Site PubMed 5 Citations 39 References
  • HAMADA Shigeyuki
    Department of Oral Microbiology and Department of Operative Dentistry, Pedodontic Clinic, Osaka University Dental School Department of Microbiology, Northwestern University Medical and Dental School
  • MASUDA Norio
    Department of Oral Microbiology and Department of Operative Dentistry, Pedodontic Clinic, Osaka University Dental School
  • OOSHIMA Takashi
    Department of Oral Microbiology and Department of Operative Dentistry, Pedodontic Clinic, Osaka University Dental School
  • SOBUE Shizuo
    Department of Oral Microbiology and Department of Operative Dentistry, Pedodontic Clinic, Osaka University Dental School
  • KOTANI Shozo
    Department of Oral Microbiology and Department of Operative Dentistry, Pedodontic Clinic, Osaka University Dental School

Bibliographic Information

Other Title
  • Epidemiological Survey of <i>Streptococcus mutans</i> among Japanese Children
  • Identification and Serological Typing of the Isolated Strains

Search this article

Description

An epidemiological investigation was carried out to identify and determine the serotypes of Streptococcus mutans from carious lesions of young Japanese children. For this purpose, a direct fluo-rescent antibody technique was mainly used. Fluorescein isothiocyanate-conjugated antibodies were prepared for the five known serotypes of S. mutans. Cross reactions and nonspecific reactions were eliminated by adsorption, counterstaining, or DEAE-cellulose column chromatography. Agar-gel immunodiffusion was used to distinguish between serotypes a and d. The epidemiological survey suggested that serotype c strains were most prevalent in dental plaques of Japanese children. The d and e serotypes were rare and serotypes a and b were not detected. It was also noted that more than one serotype of S. mutans could be found in the same locus of a carious lesion and that there might be no relationship between the degree of caries and the causative serotype(s) of S. mutans.

Journal

Citations (5)*help

See more

References(39)*help

See more

Keywords

Details 詳細情報について

Report a problem

Back to top