Chemical and Physical Properties of Lectin from Boiled Cauliflower.

DOI

Bibliographic Information

Other Title
  • ゆでカリフラワーからのレクチンの物理・化学的性質

Abstract

A lectin was purified from boiled cauliflower (Brassica oleracea var. botys) by ion-exchange chromatography on DEAE-cellulose and gel filtration on Sephacel S-1000. It was homogeneous by high-performance chromatography and its molecular weight was calculated to be 7, 400, 000. This lectin contained 67.5% carbohydrate which was mainly composed of rhamnose, mannose, galactose, glucose, arabinose and galacturonic acid. The protein fraction of this lectin was rich in alanin, glycine and glutamic acid. The lectin was resistant to proteases, and 78% of its activity remained after being heated at 100°C for 60 min. The hemagglutinating activity of this lectin was inhibited by fetuin and thyroglobulin.

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Details 詳細情報について

  • CRID
    1390282681310187776
  • NII Article ID
    130003851690
  • DOI
    10.11428/jhej1987.50.129
  • ISSN
    18820352
    09135227
  • Text Lang
    ja
  • Data Source
    • JaLC
    • CiNii Articles
  • Abstract License Flag
    Disallowed

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