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  • Ovulation in explanted hamster ovary.
  • ハムスター ノ タイガイ バイヨウ ランソウ カラ ノ ハイラン

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An improved culture method for the hamster ovary was developed to study the mechanism of ovulation. Hamsters used in this study spontaneously ovulated a mean number of 6.2 ova per ovary between 00:00 h and 02:00 h of the day of estrus. The ovaries were excised between 17:00 h of the day of proestrus and 01:00 h of the day of estrus, placed on a stainless steel wire table and cultivated at the surface of the culture medium of modified M-199 until 10:30 h of the following morning. Ova shed into the medium were counted. Among ten ovaries explanted at 17:00 h only one ovary ovulat-ed. The ovulation rate increased when the ovary was explanted at 18:00 h (47%) and 19:00 h (78%)and reached 100% at 21:00 h or later. When the ovaries were explanted at 21:00 h and 23:00 h of proestrus, the average number of shed ova was 4.7 and 4.6, respectively. These values are 75% of the value in in vivo ovulations. The ovaries explanted at 21:00 h of proestrus ovulated between 02:00 h and 06:00 h while the ovaries explanted at 00:00 h completed ovulation by 02:00 h.

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