<I>In vitro</I> metabolism of Hexestrol dicaprylate in mouse organs and its binding to estrogen receptor
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- INABA Toshio
- Department of Surgery and Obstetrics, School of Veterinary Science, College of Agriculture,University
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- SHIMIZU Ryosuke
- Department of Surgery and Obstetrics, School of Veterinary Science, College of Agriculture,University
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- IMORI Tatsuo
- Department of Surgery and Obstetrics, School of Veterinary Science, College of Agriculture,University
Bibliographic Information
- Other Title
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- 雄マウス臓器におけるヘキセストロール ジカプリレートのインビトロ代謝およびエストロジェンリセプターへの結合について
- ユウ マウス ゾウキ ニ オケル hexestrol dicaprylate
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Description
Hundred mg each of various tissues obtained from 60 days old mice was incubated with either [14C]hexestrol dicaprylate (H8; 115 nmol, 4 nCi) or [3H]-H8 (10 nmol, 0.1 μCi) at 37 C for 1 h. Following incubation, radioactive metabolites were extracted, isolated, and identified with the method of re-crystallization to constant specific activity.<BR>Only small amounts of radioactive H8 were metabolized by mouse tissues-adrenal, pancreas, intestine, fat, spleen, lung, muscle, testis, and kidney. It was observed that no radioactive H8 was metabolized by plasma.<BR>Rat uterus cytosol was incubated with either H8 or H0 and [3H]-estradiol-17 β ([3H]-E2) at 4 C for 18 h. Following incubation, [3H]-E2 binding to estrogen receptor was separated from free [3H]-E2 using dextran coated charcoal. Five nm H0 strongly competed with 0. 8 nm [3H]-E2 for binding to estrogen receptor, while 25 nm H8 weakly competed with 0.2 nm [3H]-E2.<BR>These findings will suggest that the injected H8 to animals will be slowly converted to H0 and act to the target organs after being hydrolyzed to H0.
Journal
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- The Japanese journal of animal reproduction
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The Japanese journal of animal reproduction 27 (2), 75-79, 1981
THE SOCIETY FOR REPRODUCTION AND DEVELOPMENT
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Details 詳細情報について
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- CRID
- 1390282681310940160
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- NII Article ID
- 130004213964
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- NII Book ID
- AN00041843
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- NDL BIB ID
- 2319059
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- ISSN
- 03859932
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- Text Lang
- ja
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- Data Source
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- JaLC
- NDL
- Crossref
- CiNii Articles
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- Abstract License Flag
- Disallowed