書誌事項
- タイトル別名
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- <I>In vitro</I> metabolism of Hexestrol dicaprylate in mouse organs and its binding to estrogen receptor
- ユウ マウス ゾウキ ニ オケル hexestrol dicaprylate
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説明
Hundred mg each of various tissues obtained from 60 days old mice was incubated with either [14C]hexestrol dicaprylate (H8; 115 nmol, 4 nCi) or [3H]-H8 (10 nmol, 0.1 μCi) at 37 C for 1 h. Following incubation, radioactive metabolites were extracted, isolated, and identified with the method of re-crystallization to constant specific activity.<BR>Only small amounts of radioactive H8 were metabolized by mouse tissues-adrenal, pancreas, intestine, fat, spleen, lung, muscle, testis, and kidney. It was observed that no radioactive H8 was metabolized by plasma.<BR>Rat uterus cytosol was incubated with either H8 or H0 and [3H]-estradiol-17 β ([3H]-E2) at 4 C for 18 h. Following incubation, [3H]-E2 binding to estrogen receptor was separated from free [3H]-E2 using dextran coated charcoal. Five nm H0 strongly competed with 0. 8 nm [3H]-E2 for binding to estrogen receptor, while 25 nm H8 weakly competed with 0.2 nm [3H]-E2.<BR>These findings will suggest that the injected H8 to animals will be slowly converted to H0 and act to the target organs after being hydrolyzed to H0.
収録刊行物
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- 家畜繁殖学雑誌
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家畜繁殖学雑誌 27 (2), 75-79, 1981
日本繁殖生物学会
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詳細情報 詳細情報について
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- CRID
- 1390282681310940160
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- NII論文ID
- 130004213964
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- NII書誌ID
- AN00041843
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- NDL書誌ID
- 2319059
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- ISSN
- 03859932
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- 本文言語コード
- ja
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- データソース種別
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- JaLC
- NDL
- Crossref
- CiNii Articles
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- 抄録ライセンスフラグ
- 使用不可