Epigenetic Modification Agents Improve Genomic Methylation Reprogramming in Porcine Cloned Embryos
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- HUAN Yan Jun
- Dairy Cattle Research Center, Shandong Academy of Agricultural Sciences, Jinan 250100, China College of Life Science, Northeast Agricultural University, Haerbin 150030, China
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- ZHU Jiang
- College of Life Science, Northeast Agricultural University, Haerbin 150030, China College of Animal Science and Veterinary Medicine, Shandong Agricultural University, Taian 271018, China
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- WANG Hong Mei
- Dairy Cattle Research Center, Shandong Academy of Agricultural Sciences, Jinan 250100, China
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- WU Zhan Feng
- Shouguang City Hospital of Chinese Medicine, Weifang 262700, China
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- ZHANG Ji Guang
- Shouguang City Hospital of Chinese Medicine, Weifang 262700, China
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- XIE Bing Teng
- College of Life Science, Northeast Agricultural University, Haerbin 150030, China
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- LI Jing Yu
- College of Life Science, Northeast Agricultural University, Haerbin 150030, China
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- KONG Qing Ran
- College of Life Science, Northeast Agricultural University, Haerbin 150030, China
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- LIU Zhong Hua
- College of Life Science, Northeast Agricultural University, Haerbin 150030, China
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- HE Hong Bin
- Dairy Cattle Research Center, Shandong Academy of Agricultural Sciences, Jinan 250100, China
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説明
Incomplete DNA methylation reprogramming in cloned embryos leads to low cloning efficiency. Our previous studies showed that the epigenetic modification agents 5-aza-2’-deoxycytidine (5-aza-dC) or trichostatin A (TSA) could enhance the developmental competence of porcine cloned embryos. Here, we investigated genomic methylation dynamics and specific gene expression levels during early embryonic development in pigs. In this study, our results showed that there was a typical wave of DNA demethylation and remethylation of centromeric satellite repeat (CenRep) in fertilized embryos, whereas in cloned embryos, delayed demethylation and a lack of remethylation were observed. When cloned embryos were treated with 5-aza-dC or TSA, CenRep methylation reprogramming was improved, and this was similar to that detected in fertilized counterparts. Furthermore, we found that the epigenetic modification agents, especially TSA, effectively promoted silencing of tissue specific genes and transcription of early embryo development-related genes in porcine cloned embryos. In conclusion, our results showed that the epigenetic modification agent 5-aza-dC or TSA could improve genomic methylation reprogramming in porcine cloned embryos and regulate the appropriate expression levels of genes related to early embryonic development, thereby resulting in high developmental competence.
収録刊行物
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- Journal of Reproduction and Development
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Journal of Reproduction and Development 60 (5), 377-382, 2014
公益社団法人 日本繁殖生物学会
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詳細情報 詳細情報について
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- CRID
- 1390282681313445248
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- NII論文ID
- 130004053990
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- NII書誌ID
- AA10936678
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- COI
- 1:STN:280:DC%2BC2cbks1Sltw%3D%3D
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- ISSN
- 13484400
- 09168818
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- NDL書誌ID
- 025861894
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- PubMed
- 25047549
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- 本文言語コード
- en
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- 資料種別
- journal article
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- データソース種別
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- JaLC
- NDLサーチ
- Crossref
- PubMed
- CiNii Articles
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- 使用不可