Inhibition of cathepsin B activity reduces apoptosis by preventing cytochrome c release from mitochondria in porcine parthenotes
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- KIM Seon-Hyang
- Department of Animal Sciences, Chungbuk National University, Chungbuk 361-763, Republic of Korea Brain Korea 21 Center for Bio-Resource Development, Chungbuk 361-763, Republic of Korea
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- ZHAO Ming-Hui
- Department of Animal Sciences, Chungbuk National University, Chungbuk 361-763, Republic of Korea Brain Korea 21 Center for Bio-Resource Development, Chungbuk 361-763, Republic of Korea
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- LIANG Shuang
- Department of Animal Sciences, Chungbuk National University, Chungbuk 361-763, Republic of Korea Brain Korea 21 Center for Bio-Resource Development, Chungbuk 361-763, Republic of Korea
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- CUI Xiang-Shun
- Department of Animal Sciences, Chungbuk National University, Chungbuk 361-763, Republic of Korea Brain Korea 21 Center for Bio-Resource Development, Chungbuk 361-763, Republic of Korea
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- KIM Nam-Hyung
- Department of Animal Sciences, Chungbuk National University, Chungbuk 361-763, Republic of Korea Brain Korea 21 Center for Bio-Resource Development, Chungbuk 361-763, Republic of Korea
書誌事項
- タイトル別名
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- Inhibition of cathepsin B activity reduces apoptosis by preventing cytochrome <i>c</i> release from mitochondria in porcine parthenotes
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抄録
Cathepsin B, a lysosomal cysteine protease of the papain family, has recently been implicated in the quality and developmental competence of bovine preimplantation embryos. In this study, to determine whether inhibition of cathepsin B activity can improve porcine oocyte maturation and early embryo developmental competence, we supplemented in vitro maturation or embryo culture media with E-64, a cathepsin B inhibitor. Cathepsin B activity was high in poor quality germinal vesicle stage oocytes, but no differences in mRNA expression or protein localization were observed between good and poor quality oocytes, which were categorized based on morphology. Following treatment with 1 μM E-64, cathepsin B activity sharply decreased in 4-cell and blastocyst stage embryos. E-64 had no effect on cell number but significantly (P < 0.05) increased blastocyst formation and decreased the number of apoptotic cells in blastocysts. It also significantly (P < 0.05) enhanced mitochondrial membrane potential in blastocysts, reducing the release of cytochrome c and resulting in decreased expression of Caspase-3 and Caspase-9. In conclusion, inhibition of cathepsin B activity in porcine parthenotes using 1 μM E-64 resulted in attenuation of apoptosis via a reduction in the release of cytochrome c from mitochondria.
収録刊行物
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- Journal of Reproduction and Development
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Journal of Reproduction and Development 61 (4), 261-268, 2015
公益社団法人 日本繁殖生物学会
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詳細情報 詳細情報について
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- CRID
- 1390282681313753856
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- NII論文ID
- 130005094645
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- NII書誌ID
- AA10936678
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- ISSN
- 13484400
- 09168818
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- NDL書誌ID
- 026727907
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- PubMed
- 25903788
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- 本文言語コード
- en
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- データソース種別
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- JaLC
- NDL
- Crossref
- PubMed
- CiNii Articles
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- 抄録ライセンスフラグ
- 使用不可