ネオマイシンリン酸転移酵素(neo)遺伝子を導入した胚性幹細胞(F1/1)のキメラ形成能の検討

徳永 智之, 古沢 軌, 岡崎 正幸, 林 司, 須藤 忠

doi:10.1262/jrd.38.5_j27

書誌事項

タイトル別名

Production of Chimaeric Mice Derived from Embryonic Stem Cells (F1/1) Which Have Neomycin Phosphotransferase (neo) Gene Introduced by Electroporation
ネオマイシンリンサンテンイコウソ neo イデンシオドウニュウシタハ

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説明

Non-129 embryonic stem cells (F1/1) were transformed with the neomycin phosphot-ranscerase (neo) gene by electroporation and used as a vehicle for transgenesis. There was no difference between square wave and exponential decay in transformation efficiency of F1/1 cells by selection for G418 resistance. Southern blot hybridization analysis has confirmed that 4 out of 6 neomycin resistant ES cell lines have neo gene sequences in their genomic DNA. The karyotype of these cells appeared stable, as they showed euploidy after transformation. Overt coat color chimaeras were obtained from all 4 neo resistant ES cell lines tested.

収録刊行物

Journal of Reproduction and Development

Journal of Reproduction and Development 38 (5), j27-j32, 1992

公益社団法人日本繁殖生物学会

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詳細情報詳細情報について

CRID: 1390282681314077184

NII論文ID: 130000845949

NII書誌ID: AA10936678

DOI: 10.1262/jrd.38.5_j27

ISSN: 13484400; 09168818

NDL書誌ID: 3814479

Web Site: http://id.ndl.go.jp/bib/3814479; https://ndlsearch.ndl.go.jp/books/R000000004-I3814479; http://www.jstage.jst.go.jp/article/jrd1992/38/5/38_5_j27/_pdf

本文言語コード: en

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