骨欠損部へのＢＭＰ‐１２遺伝子導入による欠損部組織の再生

DOI Web Site PubMed 参考文献26件 オープンアクセス
  • 後藤 暢大
    東京医科歯科大学大学院医歯学総合研究科口腔機能再構築学系専攻摂食機能回復学講座インプラント・口腔再生医学分野

書誌事項

タイトル別名
  • Tissue Regeneration Induced by a Transgene for Bone Morphogenetic Protein-12 (BMP-12) in a Bone Defect
  • コツ ケッソンブ エノ BMP 12 イデンシ ドウニュウ ニ ヨル ケッソンブ ソシキ ノ サイセイ
公開日
2008
資源種別
journal article
DOI
  • 10.5357/koubyou.75.29
公開者
口腔病学会

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説明

BMP-12, other than BMP-2, 4, and 7, is known to induce the formation of tendon/ligament-like tissues. However, the role of the protein in the process of bone or cartilage healing has not been adequately examined. The purpose of the present study was to examine if a plasmid vector encoding BMP-12 would induce tendon/ ligament formation in a rat bone defect model. A lyophilized atelocollagen pellet with 100μg of a plasmid vector encoding BMP-12 or BMP-2 was implanted in a bone defect of a rat femoral metaphysis. The samples were retrieved at 2, 4 and 8 weeks post-operation and subjected to histology and real-time PCR analysis. BMP-12 transgene indicated prominent fibrogenesis at 2 weeks. Consequently, the tissue was substituted by bone at 8 weeks. The gene expression of collagen type III was up-regulated by BMP-12 gene transfer after 2 weeks, followed by an increase of elastin and Sixl genes after 4 weeks. These genes, which are intimately related to tendon/ligament formation, decreased in expression at 8 weeks in preparation for osteogenesis afterward. On the other hand, the BMP-2 transgene quickly induced the synthesis of bone-related genes such as collagen type I and ALP. It is concluded that BMP-12 can initiate the development of tendon/ligament-like tissue in a rat bone defect at early healing stages. Maintaining such generation of tissue on bone could be useful for modifying periodontal tissue engineering.

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