ストロビルリン系薬剤耐性イチゴ炭疽病菌〔Colletotrichum gloeosporioides(Glomerella cingulata)〕の発生

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  • Occurrence of strobilurin-resistant strains of Colletotrichum gloeosporioides (Glomerella cingulata), the causal fungus of strawberry anthracnose.
  • ストロビルリンケイ ヤクザイ タイセイ イチゴ タンソビョウキン Colletotrichum gloeosporioides Glomerella cingulata ノ ハッセイ

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Isolates of Colletotrichum gloeosporioides (Glomerella cingulata), obtained from strawberry leaves with anthracnose in Saga Prefecture in 2003, were bioassayed for sensitivity to strobilurin fungicides (azoxystrobin and kresoxim-methyl). The two isolates had little sensitivity to these fungicides, and they were regarded as resistant. Sequence analysis showed that the deduced amino acid at codon 143 of the mitochondrial cytochrome b gene was altered from glycine (GGT) to alanine (GCT). Polymerase chain reaction-restriction fragment-length polymorphism (PCR-RFLP) using the restriction enzyme ItaI could be employed in practice for rapid testing of resistance to strobilurin fungicides.<br>

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