固定化酵素の製造　Ｖ　　放射線共重合法により製造した固定化インベルターゼの諸性質（２）

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  • Studies on the Preparation of Immobilized Enzymes by Radiopolymerization
  • コテイカ コウソ ノ セイゾウ ニカンスルケンキュウ 5 ホウシャセン キョウ
  • (第5報)放射線共重合法により製造した固定化インベルターゼの諸性質について(その2)

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The mixture of water soluble synthetic monomers and invertase solution was frozen in dry ice/acetone and radiocopolymerized by gamma-ray. The characteristics of resulting polymer which was the membranous immobilized invertase were investigated succeeding to the former report.<BR>Following results were obtained.<BR>1) There was no significant difference in the Km value between native and immobilized invertase.<BR>2) A decrease in activity of immobilized invertase was accompained by an increase in enzyme concentration and it was 8.5 to 91.5%.<BR>3) Immobilized invertase showed good stability in a dry state even if it was stored at 35°C. The residual activity was about 74 to 95% after 60 days storage and about 80% after 130 days storage.<BR>4) After the repeating usage for 30 times, immobilized enzyme showed about 70% of activity comparing to the original preparation.<BR>5) Invertase became more stable to aniline and urea when immobilized but it became more labile to ethanol, guanidin, Hg and Cu.

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