The State of Water of Myosin-gell during Dehydration

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  • 脱水ミオシンの水の状態
  • ダッスイ ミオシン ノ ミズ ノ ジョウタイ

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Abstract

State of water in dehydrated myosin was studied by dielectric dispersion measurements and differential thermal analysis. When dielectric constant and relaxation frequency were ploted as a function of Aw of dehydrated myosin with or without 0.3M sucrose, the state of water was divided to three segments demarcated by M1 and M2 which corresponded to water monolayer and water multilayer obtained from the sorption isotherm by B.E.T. and Bull's analysis. The dielectric constant of dehydrated myosin differed from that under the presence of sucrose. On the other hand, the relaxation frequency was similar to that under the presence of sucrose. This implied that the molecular rotation of water was similar in each system in spite of difference in polariza- bility of water between the two systems. In the thermograms obtained by heating from 50°to 200°C, two endothermic peaks were shown, one of which was existed below 100°C and the other was above 100°C. The latter of dehydrated myosin with sucrose was larger than that without sucrose at any tested dehydration stage. During humidification process, the increase of peak was observed only in the areas below 100°C in the case of dehydrated myosin either with or without sucrose. Hysterisis observed in sorptions might be due to the incorporation of free water which is hardly convertible to the bound water during the humidification process. From these results, it was concluded that water in the dehydrated myosin was existed in three different states. Protective effect of sucrose on the denaturation of myosin may partly be attributable to the states of water around the myosin molecule which is itself affected by the presence of sucrose.

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