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16S rDNA Genotyping of Lactic Acid Bacteria Using PCR-RFLP Analysis
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- Tamakawa Hideyuki
- Iwate industrial research institute
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- Ito Yoshihito
- Iwate industrial research institute
Bibliographic Information
- Other Title
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- PCR-RFLP法を用いた乳酸菌種16S rDNA遺伝子のジェノタイピング
- PCR-RFLPホウ オ モチイタ ニュウサン キンシュ 16S rDNA イデンシ ノ ジェノタイピング
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Description
<p>The 16S rDNA genotypes among lactic acid bacteria were determined using PCR-RFLP analysis. Eight tetrameric restriction enzymes (AccII, AfaI, HaeIII, HhaI, MboI, MspI, TaqI, XspI) were used for RFLP analysis and adequate numbers of informative band were obtained from each enzyme. Eighty-two genotypes were obtained from 99 lactic acid bacteria. In the case of only two restriction enzymes, sixty-five genotypes, which are most in all combination, were obtained from the combination of XspI and MspI restriction patterns. Therein, fifty-two species could be assigned to specific genotype, supporting the application of this analysis for identification of bacterial species. The genotype assigned by PCR-RFLP using XspI and MspI showed 92.3% successful phylogenetic affiliations. PCR-RFLP analysis using 20 lactic acid bacteria was demonstrated, and the results corresponded approximately to the calculated fragment patterns. These results indicate that PCR-RFLP should be useful for genotyping, identification and approximately classification of lactic acid bacteria.</p>
Journal
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- Nippon Shokuhin Kagaku Kogaku Kaishi
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Nippon Shokuhin Kagaku Kogaku Kaishi 64 (7), 355-364, 2017
Japanese Society for Food Science and Technology
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Details 詳細情報について
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- CRID
- 1390282681384257408
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- NII Article ID
- 130005975392
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- NII Book ID
- AN10467499
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- ISSN
- 18816681
- 1341027X
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- NDL BIB ID
- 028356826
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- Text Lang
- ja
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- Article Type
- journal article
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- Data Source
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- JaLC
- IRDB
- NDL Search
- Crossref
- CiNii Articles
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- Abstract License Flag
- Disallowed