書誌事項
- タイトル別名
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- Superoxide Anion-Scavenging Activity of Alkaline Protease Hydrolysate from Sardine Meat.
- イワシ キンニク アルカリプロテアーゼ カスイ ブンカイ ブツ ノ スーパーオキシドアニオン ショウキョ カッセイ
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Superoxide anion-scavenging activity (SOSA) of alkaline protease hydrolysate from sardine meat (A-1) was measured by colorimetry using water-soluble tetrazolium salt (WST-1). A-1 showed low activity with IC50 value of 9.21mg powder/ml. The hydrolysate was separated into F-1-F-6 on an ODS column. The most active fraction (F-4) was fractionated by ion exchange chromatography on SP-Sephadex C-25 into three active fractions (1/SP-3/SP). The first fraction, 1/SP, showed the most potent activity (IC50=4.11mg powder/ml). By elution with a stepwise gradient of acetonitrile containing 0.1% TFA on Sep-pak plus C18 cartridge, A/SE (IC50=2.12mg powder/ml) and B/SE (IC50=7.14mg powder/ml) were obtained from 1/SP. A/SE was extracted with water or 20% acetonitrile. A/SE W, the water extract, showed high SOSA with an IC50 value of 0.51mg powder/ml. The amino acid analysis of each active fraction indicated that five amino acid residues (Leu, Phe, Pro, Met, Tyr) played important roles in the activity. Moreover, it was suggested that the potent scavenger contained few histidine residue.
収録刊行物
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- 日本食品科学工学会誌
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日本食品科学工学会誌 50 (9), 392-398, 2003
公益社団法人 日本食品科学工学会
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詳細情報 詳細情報について
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- CRID
- 1390282681385520640
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- NII論文ID
- 10011626880
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- NII書誌ID
- AN10467499
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- ISSN
- 18816681
- 1341027X
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- NDL書誌ID
- 6708726
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- 本文言語コード
- ja
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- データソース種別
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- JaLC
- NDL
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- 使用不可