Characterization of Anti-peptide Antibody for Wheat Nonspecific Lipid Transfer Protein 1
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- Oita Shigeru
- National Agricultural Research Center for Tohoku Region
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- Yokota Satoshi
- National Agricultural Research Center for Hokkaido Region
Bibliographic Information
- Other Title
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- コムギNonspecific Lipid Transfer Protein 1抗ペプチド抗体の特性
- コムギ Nonspecific Lipid Transfer Protein 1 コウペプチド コウタイ ノ トクセイ
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Abstract
To obtain a highly specific immunoglobulin G (IgG) for wheat nonspecific lipid transfer protein 1 (LTP), a wheat LTP peptide comprising amino acids Nos. 17-27 was designed as the antigen. Rabbit IgG showed high affinity to LTP of wheat and rye, but less or no affinity to water-extracted proteins of barley, oat, rice and maize. The LTP in 1μg of water-extracted wheat and rye protein was detectable by immunoblotting. Purified wheat LTP of over 2ng was detectable by immunoblotting and enzyme linked immunosorbent assay.
Journal
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- Nippon Shokuhin Kagaku Kogaku Kaishi
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Nippon Shokuhin Kagaku Kogaku Kaishi 55 (4), 137-142, 2008
Japanese Society for Food Science and Technology
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Details 詳細情報について
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- CRID
- 1390282681385939200
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- NII Article ID
- 10021178454
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- NII Book ID
- AN10467499
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- COI
- 1:CAS:528:DC%2BD1cXmtVSlt7w%3D
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- ISSN
- 18816681
- 1341027X
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- NDL BIB ID
- 9455559
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- Text Lang
- ja
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- Data Source
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- JaLC
- NDL
- Crossref
- CiNii Articles
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- Abstract License Flag
- Disallowed