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- 小川 幸春
- 米国農務省西部地域研究所
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- Wood Delilah F.
- U. S. Department of Agriculture, ARS, Western Regional Research Center
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- Orts William J.
- U. S. Department of Agriculture, ARS, Western Regional Research Center
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- Glenn Gregory M.
- U. S. Department of Agriculture, ARS, Western Regional Research Center
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- 宮下 一成
- 茨城大学大学院農学研究科
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- 蔦 瑞樹
- 東京大学大学院農学生命科学研究科
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- 杉山 純一
- 独立行政法人食品総合研究所
書誌事項
- タイトル別名
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- Observation Method for the Histological Structure of Cooked Rice Kernels Using Adhesive Tape
- ネンチャク テープ オ リヨウ シタ カンイ セッペンカ ニ ヨル ベイハンリュウ ノ ソシキ コウゾウ カンサツホウ
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説明
A new method was developed to investigate histological structures of cooked rice kernels. A single kernel of rice was infiltrated with 100% ethanol by carrying the kernel through a graded series of ethanol-water mixtures. The rice kernel was then embedded in paraffin and sectioned on a standard microtome. During the sectioning process, a special adhesive tape was used to collect the sections and place them onto slides so that they remained flat and were observable by microscope. The quality of sections was shown using scanning electron microscope. The use of the adhesive tape technique combined with autofluorescence characteristics of phenolic compounds located in cell walls in the UV range allowed cell formations to be visualized throughout an entire longitudinal section of a rice kernel. Cell wall disruptions in the sections of the cooked rice kernel were also visualized. This technique shows potential for studying histological characteristics of cooked rice kernels.
収録刊行物
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- 日本食品科学工学会誌
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日本食品科学工学会誌 50 (7), 319-323, 2003
公益社団法人 日本食品科学工学会
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キーワード
詳細情報 詳細情報について
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- CRID
- 1390282681386945152
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- NII論文ID
- 10011626664
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- NII書誌ID
- AN10467499
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- ISSN
- 18816681
- 1341027X
- http://id.crossref.org/issn/1341027X
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- NDL書誌ID
- 6648205
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- 本文言語コード
- ja
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- データソース種別
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- JaLC
- NDL
- Crossref
- CiNii Articles
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- 抄録ライセンスフラグ
- 使用不可