ホタテガイ閉殻筋からの筋原繊維の調製とその生化学的性質

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タイトル別名
  • Preparation and Biochemical Study of Myofibrils from Adductor Muscle of Scallop
  • ホタテガイ ヘイカク キン カラ ノ キンゲン センイ ノ チョウセイ ト ソ

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Attempts were made to prepare the myofibrils from the striated and smooth adductor muscles of giant ezo scallop. Patinopecten yessoensis. In order to eliminate the membraneous contaminants, myofibrils were washed with a buffer solution containing 1% Triton X-100.<br>(1) By phase-contrast microscopy, the striated structure was evident in the myofibrils from the striated adductor muscles but those from the smooth adductor muscles showed a vague feature with a relatively larger size than the former.<br>(2) Both myofibril preparations, which were proved homogeneous, represented essentially the same ATPase activity profiles, such as activation by Ca2+ as well as Mg2+, KCl dependency, activation by urea, and Ca-sensitivity. The measurement of inhibition of myofibrillar ATPase by azide, ouabain or quinidine concluded that the contamination of the membraneous ATPase in these myofibril preparations was negligible.<br>(3) Comparison of the first order rate constants for the inactivation of myofibrillar Ca2+ ATPase at 35°C showed that the stability of both myofibrils seemed to be identical.

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  • 日本水産学会誌

    日本水産学会誌 44 (6), 587-593, 1978

    公益社団法人 日本水産学会

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