Denaturation of alaska pollack myosin B induced by NaCl.
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- Nishimoto Shin-ichiro
- Laboratory of Biochemistry, Faculty of Fisheries, Hokkaido University
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- Arai Ken-ichi
- Laboratory of Biochemistry, Faculty of Fisheries, Hokkaido University
Bibliographic Information
- Other Title
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- スケトウダラ・ミオシンBのNaClによる変性
- スケトウダラ ミオシン B ノ NaCl ニ ヨル ヘンセイ
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Abstract
Alaska pollack myosin B (MB) was dissolved in 40mM Tris-maleate (pH 7.0) containing varied concentrations (0.3-2.0M) of NaCl and stored at 10°C, to examine for physico-chemical and bio-chemical changes. At 1.0M or higher concentrations of NaCl, MB showed a marked decrease in viscosity and ATP-sensitivity, a decrease in Mg-, and EDTA-ATPase activities. These changes were accompanied by a concomitant appearance of myosin in ultracentrifugal and gel filtration patterns. Occurrence of 50mM KCl-soluble actin was also observed. The soluble actin did not activate Mg-ATPase activity nor inhibit EDTA-ATPase activity of intact myosin. On the other hand, MB quickly lost Ca-ATPase activity when treated with NaCl more than 1.0M.<br> These results indicated that most of Alaska pollack MB was dissociated into myosin and actin, and both components were denatured at higher concentration of NaCl.
Journal
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- NIPPON SUISAN GAKKAISHI
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NIPPON SUISAN GAKKAISHI 54 (8), 1429-1436, 1988
The Japanese Society of Fisheries Science
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Keywords
Details 詳細情報について
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- CRID
- 1390282681391583616
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- NII Article ID
- 130001544530
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- NII Book ID
- AN00193422
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- ISSN
- 1349998X
- 00215392
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- NDL BIB ID
- 3196398
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- Text Lang
- ja
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- Data Source
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- JaLC
- IRDB
- NDL
- Crossref
- CiNii Articles
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- Abstract License Flag
- Disallowed