南極産オキアミの蛋白質分解酵素について

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書誌事項

タイトル別名
  • 南極産オキアミのたんぱく質分解酵素
  • Studies on Proteases from Antarctic Krill
  • ナンキョクサン オキアミ ノ タンパクシツ ブンカイ コウソ ニ ツイテ

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説明

Proteases were extracted from antarctic krill, Euphausia superba, with borate buffer at pH 8.0 They were partially purified by ammonium sulfate fractionation and gel filtration through Sephadex G-100. Further. purification performed with chromatohraphy on DEAE-Sephadex A-50 separated them into two trypsin-like proteases and a protease of unknown specificity. The trypsin-like proteases were characterized by their hydrolysis of TAME as well as the inhibition of the activity in the presence of soy-bean trypsin inhibitor. However, the inhibition of the two tryptic activities by PMSF was less than that in the case of bovine trypsin.<br> All three proteases showed optimal activity at the physiological pH (around 8.0) of krill body fluids. They were stable only in an alkaline medium and digested carp actomyosin. These results suggest that they are responsible for the rapid autolysis of krill body protein which occurs after catching.

収録刊行物

  • 日本水産学会誌

    日本水産学会誌 43 (8), 955-962, 1977

    公益社団法人 日本水産学会

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