Actinobacillus (Haemophilus) pleuropneumoniae血清型2・菌株に対するモノクローナル抗体の作製とその性状
書誌事項
- タイトル別名
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- Production and characterization of monoclonal antibodies against Actinobacillus (Haemophilus) pleuropneumoniae serotype 2.
- Actinobacillus(Haemophilus)pleuropneumoniae血清型2・菌株に対するモノクローナル抗体の作製とその性状〔英文〕
- Actinobacillus Haemophilus pleuropneumo
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説明
Four hybridoma cell lines producing monoclonal antibodies (MAbs) against Actinobacillus (Haemophilus) pleuropneumoniae were established by fusion of mouse myeloma and spleen cells obtained from mice immunized with a serotype 2, strain SH-15. Enzyme-linked immunosorbent assay-inhibition tests with antigens obtained from 12 serotype strains of A. pleuropneumoniae and 9 other gram-negative bacteria showed that all the MAbs bound to only serotype 2 strains of A. pleuropneumoniae. The epitopes recognized by the MAbs were located on a carbohydrate moiety of lipopolysaccharide (LPS) of the organism, which was sensitive to periodate oxidation. In immunoblotting analyses of LPS obtained from A. pleuropneumoniae serotype 2, all the four MAbs reacted specifically with the characteristic ladder bands of LPS detected by sodium dodecyl sulfate-polyacrylamide gel electrophoresis. These results suggest that O-antigen side chains of the LPS are one of the antigenic determinants responsible for the serotype-specificity of A. pleuropneumoniae.
収録刊行物
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- 日本獸醫學雜誌(The Japanese Journal of Veterinary Science)
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日本獸醫學雜誌(The Japanese Journal of Veterinary Science) 52 (3), 533-542, 1990
公益社団法人 日本獣医学会
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詳細情報 詳細情報について
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- CRID
- 1390282681399009408
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- NII論文ID
- 110003918227
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- NII書誌ID
- AN00191788
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- COI
- 1:STN:280:By%2BA2cfgsFc%3D
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- ISSN
- 18811442
- 00215295
- http://id.crossref.org/issn/00215295
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- NDL書誌ID
- 3670891
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- PubMed
- 2385034
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- 本文言語コード
- en
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- データソース種別
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- JaLC
- IRDB
- NDL
- Crossref
- PubMed
- CiNii Articles
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- 抄録ライセンスフラグ
- 使用不可