Thyroid Hormone Augments GLUT4 Expression and Insulin-Sensitive Glucose Transport System in Differentiating Rat Brown Adipocytes in Culture.
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- SHIMIZU Yasutake
- Department of Medical Biochemistry, Ehime University School of Medicine
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- SHIMAZU Takashi
- Department of Medical Biochemistry, Ehime University School of Medicine
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Description
The effects of triiodothyronine (T3) on differentiation-dependent expression of GLUT and responses of glucose transport to insulin and norepinephrine (NE) were investigated. Precursor cells of brown adipocytes isolated from the interscapular brown adipose tissue of newborn rats were cultured in the absence or presence of various concentrations of T3. Western bolt analysis revealed that treatment with T3 resulted in an increased expression of GLUT4, in a dose-dependent manner, whereas GLUT1 contents were unchanged. In parallel with the increase in GLUT4 expression, T3 improved insulin sensitivity for glucose transport, being accompanied by an increase in maximal transport rate and a reduction of ED50. In contrast, T3-treatment of the brown adipocytes during the differentiation process had little effect on NE-regulatable glucose transport system. These results suggest that T3 plays a predominant role in the development of insulin-sensitive glucose transport during differentiation of brown adipocytes.
Journal
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- Journal of Veterinary Medical Science
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Journal of Veterinary Medical Science 64 (8), 677-681, 2002
JAPANESE SOCIETY OF VETERINARY SCIENCE
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Details 詳細情報について
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- CRID
- 1390282681402706432
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- NII Article ID
- 130000449242
- 110003920897
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- NII Book ID
- AA10796138
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- COI
- 1:CAS:528:DC%2BD38XnsFOiu74%3D
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- ISSN
- 13477439
- 09167250
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- NDL BIB ID
- 6275412
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- PubMed
- 12237511
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- Text Lang
- en
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- Data Source
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- JaLC
- NDL
- Crossref
- PubMed
- CiNii Articles
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- Abstract License Flag
- Disallowed