Generation of Neuronal-Like Cells from Umbilical Cord Blood-Derived Mesenchymal Stem Cells of a RFP-Transgenic Cloned Cat

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  • JIN Guang-Zhen
    Division of Applied Life Science (BK21), Graduate School of Gyeongsang National University
  • YIN Xi-Jun
    Department of Animal Science & Technology, Sunchon National University
  • YU Xian-Feng
    Department of Animal Science & Technology, Sunchon National University
  • CHO Su-Jin
    Division of Applied Life Science (BK21), Graduate School of Gyeongsang National University
  • CHOI Eu-Gene
    Division of Applied Life Science (BK21), Graduate School of Gyeongsang National University
  • LEE Young-S
    Division of Applied Life Science (BK21), Graduate School of Gyeongsang National University
  • JEON Jin-Tae
    Division of Applied Life Science (BK21), Graduate School of Gyeongsang National University
  • YEE Sung-Tae
    Department of Biology, Sunchon National University
  • KONG Il-Keun
    Division of Applied Life Science (BK21), Graduate School of Gyeongsang National University Institute of Agriculture and Life Science, Graduate School of Gyeongsang National University

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  • Laboratory animal science: Generation of neuronal-like cells from umbilical cord blood-derived mesenchymal stem cells of a RFP-transgenic cloned cat

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Abstract

Umbilical cord blood (UCB)-derived mesenchymal stem cells (MSCs) are multipotent adult stem cells, which can differentiation into cells of connective tissue and neural lineages. This study investigated the potential for neuronal differentiation of red fluorescent protein (RFP)-transgenic cat UCB-derived MSCs. The cells were cultured in pre-induction medium for 24 hr and in neuronal-induction medium for 72 hr. Immunofluorescent staining showed that 6.85% of the total cells were β III-tubulin-positive, 3.37% were neurofilament light (NF-L)-positive and 7.04% were neurofilament medium (NF-M)-positive. A β III-tubulin band was detected by western blot analysis. Our results demonstrate that RFP-transgenic UCB-derived MSCs can be differentiated into neuronal cells in vitro. Thus, RFP-transgenic MSCs could provide alternative tracing material for stem cell transplantation.<br>

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