Laboratory animal science: Generation of neuronal-like cells from umbilical cord blood-derived mesenchymal stem cells of a RFP-transgenic cloned cat
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- JIN Guang-Zhen
- Division of Applied Life Science (BK21), Graduate School of Gyeongsang National University
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- YIN Xi-Jun
- Department of Animal Science & Technology, Sunchon National University
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- YU Xian-Feng
- Department of Animal Science & Technology, Sunchon National University
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- CHO Su-Jin
- Division of Applied Life Science (BK21), Graduate School of Gyeongsang National University
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- CHOI Eu-Gene
- Division of Applied Life Science (BK21), Graduate School of Gyeongsang National University
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- LEE Young-S
- Division of Applied Life Science (BK21), Graduate School of Gyeongsang National University
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- JEON Jin-Tae
- Division of Applied Life Science (BK21), Graduate School of Gyeongsang National University
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- YEE Sung-Tae
- Department of Biology, Sunchon National University
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- KONG Il-Keun
- Division of Applied Life Science (BK21), Graduate School of Gyeongsang National University Institute of Agriculture and Life Science, Graduate School of Gyeongsang National University
書誌事項
- タイトル別名
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- Generation of Neuronal-Like Cells from Umbilical Cord Blood-Derived Mesenchymal Stem Cells of a RFP-Transgenic Cloned Cat
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抄録
Umbilical cord blood (UCB)-derived mesenchymal stem cells (MSCs) are multipotent adult stem cells, which can differentiation into cells of connective tissue and neural lineages. This study investigated the potential for neuronal differentiation of red fluorescent protein (RFP)-transgenic cat UCB-derived MSCs. The cells were cultured in pre-induction medium for 24 hr and in neuronal-induction medium for 72 hr. Immunofluorescent staining showed that 6.85% of the total cells were β III-tubulin-positive, 3.37% were neurofilament light (NF-L)-positive and 7.04% were neurofilament medium (NF-M)-positive. A β III-tubulin band was detected by western blot analysis. Our results demonstrate that RFP-transgenic UCB-derived MSCs can be differentiated into neuronal cells in vitro. Thus, RFP-transgenic MSCs could provide alternative tracing material for stem cell transplantation.<br>
収録刊行物
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- The Journal of Veterinary Medical Science
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The Journal of Veterinary Medical Science 70 (7), 723-726, 2008
公益社団法人 日本獣医学会
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詳細情報 詳細情報について
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- CRID
- 1390282681406556032
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- NII論文ID
- 110006839596
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- NII書誌ID
- AA10796138
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- COI
- 1:STN:280:DC%2BD1crgtlSksQ%3D%3D
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- ISSN
- 13477439
- 09167250
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- NDL書誌ID
- 9591613
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- 本文言語コード
- en
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- データソース種別
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- JaLC
- NDL
- Crossref
- CiNii Articles
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- 抄録ライセンスフラグ
- 使用不可