Practical Inoculation Site for Experimental Infection of Japanese Flounder Paralichthys olivaceus with Streptococcus parauberis

DOI JASI Web Site 17 References
  • Mori Kyoko
    Fisheries Research Institute, Oita Prefectural Agriculture, Forestry and Fisheries Research Center
  • Fukuda Yutaka
    Fisheries Research Institute, Oita Prefectural Agriculture, Forestry and Fisheries Research Center
  • Togo Arisa
    Laboratory of Fish Health Management, Tokyo University of Marine Science and Technology
  • Miyoshi Yasuyuki
    Fisheries Research Institute, Oita Prefectural Agriculture, Forestry and Fisheries Research Center
  • Endo Makoto
    Laboratory of Fish Health Management, Tokyo University of Marine Science and Technology

Bibliographic Information

Other Title
  • ヒラメのＳｔｒｅｐｔｏｃｏｃｃｕｓ　ｐａｒａｕｂｅｒｉｓ実験感染における接種部位の検討
  • ヒラメ ノ Streptococcus parauberis ジッケン カンセン ニ オケル セッシュ ブイ ノ ケントウ

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Abstract

In experimental infection with Streptococcus parauberis in Japanese flounder Paralichthys olivaceus, we investigated suitable routes of inoculation for a reproduction of natural infection: intraperitoneal injection, intravascular one in tail, intramuscular one into a lateral muscle or erecting and depressing muscles of dorsal fin, and subcutaneous one at a base of dorsal fin or outside preopercular bone. Among these routes, the subcutaneous injection resulted in the challenge-dose-dependent mortality. Disease and histopathological signs of the dead fish were similar to those of spontaneously affected fish. The subcutaneous injection at a base of dorsal fin or outside preopercular bone is a recommended method at a challenge of S. parauberis.

Journal

  • Fish Pathology

    Fish Pathology 45 (1), 37-42, 2010

    The Japanese Society of Fish Pathology

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